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Revision as of 15:09, 14 November 2010
Whole-Genome Random Sequencing and Assembly of Haemophilus Influenzae Rd
Fleischmann, Robert D.; Adams, Mark D.; White, Owen; Clayton, Rebecca A.; Kirkness, Ewen F.; Kerlavage, Anthony R.; Bult, Carol J.; Tomb, Jean-Francois; Dougherty, Brian A.; Merrick, Joseph M.; McKenney, Keith; Sutton, Granger; Fitzhugh, Will; Fields, Chris; Gocyne, Jeannie D.; Scott, John; Shirley, Robert; Liu, Li-Ing; Glodek, Anna; Kelley, Jenny M.; Weidman, Janice F.; Phillips, Cheryl A.; Spriggs, Tracy; Hedblom, Eva; Cotton, Matthew D.; Utterback, Teresa R.; Hanna, Michael C.; Nguyen, David T.; Saudek, Deborah M.; Brandon, Rhonda C.; Fine, Leah D.; Fritchman, Janice L.; Fuhrmann, Joyce L.; Geoghagen, N. S. M.; Gnehm, Cheryl L.; McDonald, Lisa A.; Small, Keith V.; Fraser, Claire M.; Smith, Hamilton O.; Venter, J. Craig
An approach for genome analysis based on sequencing and assembly of unselected pieces of DNA from the whole chromosome has been applied to obtain the complete nucleotide sequence (1,830, 137 base pairs) of the genome from the bacterium Haemophilus influenzae Rd. This approach eliminates the need for initial mapping efforts and is therefore applicable to the vast array of microbial species for which genome maps are unavailable. The H. influenzae Rd genome sequence (Genome Sequence DataBase accession number L42023) represents the only complete genome sequence from a free-living organism.
Science, Volume 269, Issue 5223, pp. 496-498,507-512. DOI: 10.1126/science.7542800
