View source for Method of Sample (gDNA) Preparation for Sending out

<h1><span id="1..C2.A0Sample.28gDNA.29_Preparation" class="mw-headline">1.&nbsp;Sample(gDNA) Preparation</span></h1>
<p>&nbsp;</p>
<p>1)&nbsp;&nbsp;DNA should be dissolved in TE buffer (Tris 10mM pH 8,0, 0.1 mM EDTA) or 10 mM Tris pH 8.0.</p>
<p>2)&nbsp;&nbsp;Do Not Dissolve in Water</p>
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<h1><span id="2..C2.A0Sample.28gDNA.29_Requirement" class="mw-headline">2.&nbsp;Sample(gDNA) Requirement</span></h1>
<p>&nbsp;</p>
<p>1)&nbsp;&nbsp;&nbsp;Amount and concentration spec.&nbsp;: over 20 ug of gDNA with &gt;50 ng/ul</p>
<p>&nbsp;This amount is required for</p>
<p>a.&nbsp;&nbsp;Whole genome sequencing (30X)</p>
<p>b.&nbsp;&nbsp;Whole exome sequencing (50X)</p>
<p>c.&nbsp;&nbsp;Genotyping</p>
<p>2)&nbsp;&nbsp;Sample(gDNA) should be&nbsp;: OD260/280=1.8~2.0; free of protein, RNA, or other visible contamination</p>
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<h1><span id="3..C2.A0Sample.28gDNA.29_Packing_Information.C2.A0" class="mw-headline">3.&nbsp;Sample(gDNA) Packing Information&nbsp;</span></h1>
<p>&nbsp;</p>
<p>This way is only available when DNA is dissolved in TE or Tris buffer.</p>
<p>1)&nbsp;&nbsp;If not, please pack DNA with icepack in icebox and use Fedex or DHL service</p>
<p>2)&nbsp;&nbsp;Mark sample information on tube (concentration, sample name&hellip;what you want)</p>
<p>3)&nbsp;&nbsp;Seal the cap of tube with parafilm well</p>
<p>4)&nbsp;&nbsp;Wrap the tube with paper towel or equivalent</p>
<p>5)&nbsp;&nbsp;Put into the 50 ml Falcon tube and close the cap</p>
<p>6)&nbsp;&nbsp;Send the sample with air cushion envelop by FEDEX or DHL or equivalent</p>
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<h1><span id="4..C2.A0Requirement_of_Sample.28gDNA.29_Information" class="mw-headline">4.&nbsp;Requirement of Sample(gDNA) Information</span></h1>
<p>&nbsp;</p>
<p>1)&nbsp; Sample type: DNA</p>
<p>2)&nbsp; Condition: DNA in TE buffer or EB buffer or equivalent. Not in water</p>
<p>3)&nbsp; Gel image of DNA electrophoresis</p>
<p>4)&nbsp; Species</p>
<p>5)&nbsp; No. of Tubes</p>
<p>6)&nbsp; Concentration (ng/&mu;l)</p>
<p>7)&nbsp; Volume (&mu;l)</p>
<p>8) &nbsp;Total Quantity (&mu;g)</p>
<p>9)&nbsp; OD260/280</p>
<p>10)&nbsp; OD260/230</p>
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<p><b><span style="font-size: medium">Please download and refer to the following documents for information on gDNA methods of preparation and delivery.</span></b></p>
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<p>1.<span style="color: #800000"><a href="/userfiles/Method of Sample(gDNA) Preparation for Sending out.doc">Method of Sample (gDNA) Preparation for Sending out</a></span></p>
<p>2.<span style="color: #800000"><a href="/userfiles/Declaration_of_Shipment_for_anyone(1).doc">Declaration of Shipment</a><br />
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